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cd19 microbeads  (Miltenyi Biotec)


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    Miltenyi Biotec cd19 microbeads
    Cd19 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd19+selection+kit/MACSprep+CD3+and+CD19+Selection+Kit%2C+human/pmc13114487-50-16-18
    Average 94 stars, based on 2 article reviews
    cd19 microbeads - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Selection:

    Article Title: EACR 2025 Congress: Innovative Cancer Science, 16-19 June 2025.
    Article Snippet: .. With the MACSprepTM CD3 and CD19 Selection Kit, Miltenyi Biotec offers a new cell separation workflow to generate T and B cells from limited human blood samples in manual and automated processes. ..

    Amplification:

    Article Title: B cell receptor ligation induces display of V-region peptides on MHC class II molecules to T cells
    Article Snippet: .. Lambda amplicon sequencing of B cells from the V2315m mouse B cells were positively sorted from the spleens of a young naïve heterozygous V2 315m mouse using a CD19-selection kit (Miltenyi) and total RNA was isolated using an RNA Easy Kit (Qiagen). .. 5 μl RNA was used for cDNA generation using kit (K1612, Thermo Scientific). cDNA quality was evaluated using primers for GAPDH included in the kit.

    Sequencing:

    Article Title: B cell receptor ligation induces display of V-region peptides on MHC class II molecules to T cells
    Article Snippet: .. Lambda amplicon sequencing of B cells from the V2315m mouse B cells were positively sorted from the spleens of a young naïve heterozygous V2 315m mouse using a CD19-selection kit (Miltenyi) and total RNA was isolated using an RNA Easy Kit (Qiagen). .. 5 μl RNA was used for cDNA generation using kit (K1612, Thermo Scientific). cDNA quality was evaluated using primers for GAPDH included in the kit.

    Isolation:

    Article Title: B cell receptor ligation induces display of V-region peptides on MHC class II molecules to T cells
    Article Snippet: .. Lambda amplicon sequencing of B cells from the V2315m mouse B cells were positively sorted from the spleens of a young naïve heterozygous V2 315m mouse using a CD19-selection kit (Miltenyi) and total RNA was isolated using an RNA Easy Kit (Qiagen). .. 5 μl RNA was used for cDNA generation using kit (K1612, Thermo Scientific). cDNA quality was evaluated using primers for GAPDH included in the kit.



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    (A) Representative contour plots and cumulative data showing the frequencies of <t>CD19</t> + CD27 - IgD - (double negative (DN)), CD19 + CD27 + IgD - , CD19 + CD27 + IgD + (unswitched memory (USM)) and CD19 + CD27 - IgD + (naive) B cells ex vivo in paired BK and matching tumor tissue; ** p <0.01, *** p <0.001. n= 32 biologically independent samples. DN, USM, and naive B cells analyzed by paired two-tailed Student’s t -test, CD19 + CD27 + IgD - B cells analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (B) Representative contour plots and cumulative data showing the frequencies of CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) and BLIMP-1 + B cells ex vivo in paired BK and matching tumor tissue. BLIMP-1 expression overlaid onto the B cell contour plot; ** p <0.01, **** p <0.0001. For plasma cells n =31 and for BLIMP-1 + B cells n =15 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (C) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 + IgD - CD24 + CD38 - IgM + (IgM + memory) and CD19 + CD27 + IgD - CD24 + CD38 - IgM - (class-switched memory (CSM)) B cells ex vivo in paired BK and tumor; *** p <0.001. n= 32 biologically independent samples. CSM analyzed by paired two-tailed Student’s t -test, IgM + memory analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (D) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD + CD24 int CD38 int (mature) and CD19 + CD27 - IgD + CD24 hi CD38 hi transitional B cells ex vivo in paired BK and tumor. n= 32 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (E) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c + (DN2), CD19 + CD27 - IgD - CD21 - CD11c - (DN3), and CD19 + CD27 - IgD - CD21 + CD11c + (DN4) B cells ex vivo in BK and tumor; ** p <0.01. n= 18 biologically independent samples. Paired two-tailed Student’s t -test. Error bars represented as mean±SEM. (F) Cumulative data showing the frequencies of CD19 + CD27 - IgD + (naive), CD19 + CD27 + IgD - , CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c - (DN3) B cells, and CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) stratified according to intermediate (3-5) or high (>6) Leibovich scores; * p <0.05, ** p <0.01. For naive, CD27 + IgD - , and plasma cells n= 26, for DN1 and DN3 n= 13 biologically independent samples. Naive, DN1, DN3, and PC analyzed by unpaired two-tailed Student’s t -test, CD27 + IgD - analyzed by unpaired Mann-Whitney U test. Error bars represented as mean±SEM.
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    (A) Representative contour plots and cumulative data showing the frequencies of <t>CD19</t> + CD27 - IgD - (double negative (DN)), CD19 + CD27 + IgD - , CD19 + CD27 + IgD + (unswitched memory (USM)) and CD19 + CD27 - IgD + (naive) B cells ex vivo in paired BK and matching tumor tissue; ** p <0.01, *** p <0.001. n= 32 biologically independent samples. DN, USM, and naive B cells analyzed by paired two-tailed Student’s t -test, CD19 + CD27 + IgD - B cells analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (B) Representative contour plots and cumulative data showing the frequencies of CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) and BLIMP-1 + B cells ex vivo in paired BK and matching tumor tissue. BLIMP-1 expression overlaid onto the B cell contour plot; ** p <0.01, **** p <0.0001. For plasma cells n =31 and for BLIMP-1 + B cells n =15 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (C) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 + IgD - CD24 + CD38 - IgM + (IgM + memory) and CD19 + CD27 + IgD - CD24 + CD38 - IgM - (class-switched memory (CSM)) B cells ex vivo in paired BK and tumor; *** p <0.001. n= 32 biologically independent samples. CSM analyzed by paired two-tailed Student’s t -test, IgM + memory analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (D) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD + CD24 int CD38 int (mature) and CD19 + CD27 - IgD + CD24 hi CD38 hi transitional B cells ex vivo in paired BK and tumor. n= 32 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (E) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c + (DN2), CD19 + CD27 - IgD - CD21 - CD11c - (DN3), and CD19 + CD27 - IgD - CD21 + CD11c + (DN4) B cells ex vivo in BK and tumor; ** p <0.01. n= 18 biologically independent samples. Paired two-tailed Student’s t -test. Error bars represented as mean±SEM. (F) Cumulative data showing the frequencies of CD19 + CD27 - IgD + (naive), CD19 + CD27 + IgD - , CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c - (DN3) B cells, and CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) stratified according to intermediate (3-5) or high (>6) Leibovich scores; * p <0.05, ** p <0.01. For naive, CD27 + IgD - , and plasma cells n= 26, for DN1 and DN3 n= 13 biologically independent samples. Naive, DN1, DN3, and PC analyzed by unpaired two-tailed Student’s t -test, CD27 + IgD - analyzed by unpaired Mann-Whitney U test. Error bars represented as mean±SEM.
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    (A) Representative contour plots and cumulative data showing the frequencies of <t>CD19</t> + CD27 - IgD - (double negative (DN)), CD19 + CD27 + IgD - , CD19 + CD27 + IgD + (unswitched memory (USM)) and CD19 + CD27 - IgD + (naive) B cells ex vivo in paired BK and matching tumor tissue; ** p <0.01, *** p <0.001. n= 32 biologically independent samples. DN, USM, and naive B cells analyzed by paired two-tailed Student’s t -test, CD19 + CD27 + IgD - B cells analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (B) Representative contour plots and cumulative data showing the frequencies of CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) and BLIMP-1 + B cells ex vivo in paired BK and matching tumor tissue. BLIMP-1 expression overlaid onto the B cell contour plot; ** p <0.01, **** p <0.0001. For plasma cells n =31 and for BLIMP-1 + B cells n =15 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (C) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 + IgD - CD24 + CD38 - IgM + (IgM + memory) and CD19 + CD27 + IgD - CD24 + CD38 - IgM - (class-switched memory (CSM)) B cells ex vivo in paired BK and tumor; *** p <0.001. n= 32 biologically independent samples. CSM analyzed by paired two-tailed Student’s t -test, IgM + memory analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (D) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD + CD24 int CD38 int (mature) and CD19 + CD27 - IgD + CD24 hi CD38 hi transitional B cells ex vivo in paired BK and tumor. n= 32 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (E) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c + (DN2), CD19 + CD27 - IgD - CD21 - CD11c - (DN3), and CD19 + CD27 - IgD - CD21 + CD11c + (DN4) B cells ex vivo in BK and tumor; ** p <0.01. n= 18 biologically independent samples. Paired two-tailed Student’s t -test. Error bars represented as mean±SEM. (F) Cumulative data showing the frequencies of CD19 + CD27 - IgD + (naive), CD19 + CD27 + IgD - , CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c - (DN3) B cells, and CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) stratified according to intermediate (3-5) or high (>6) Leibovich scores; * p <0.05, ** p <0.01. For naive, CD27 + IgD - , and plasma cells n= 26, for DN1 and DN3 n= 13 biologically independent samples. Naive, DN1, DN3, and PC analyzed by unpaired two-tailed Student’s t -test, CD27 + IgD - analyzed by unpaired Mann-Whitney U test. Error bars represented as mean±SEM.
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    (A) Representative contour plots and cumulative data showing the frequencies of <t>CD19</t> + CD27 - IgD - (double negative (DN)), CD19 + CD27 + IgD - , CD19 + CD27 + IgD + (unswitched memory (USM)) and CD19 + CD27 - IgD + (naive) B cells ex vivo in paired BK and matching tumor tissue; ** p <0.01, *** p <0.001. n= 32 biologically independent samples. DN, USM, and naive B cells analyzed by paired two-tailed Student’s t -test, CD19 + CD27 + IgD - B cells analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (B) Representative contour plots and cumulative data showing the frequencies of CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) and BLIMP-1 + B cells ex vivo in paired BK and matching tumor tissue. BLIMP-1 expression overlaid onto the B cell contour plot; ** p <0.01, **** p <0.0001. For plasma cells n =31 and for BLIMP-1 + B cells n =15 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (C) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 + IgD - CD24 + CD38 - IgM + (IgM + memory) and CD19 + CD27 + IgD - CD24 + CD38 - IgM - (class-switched memory (CSM)) B cells ex vivo in paired BK and tumor; *** p <0.001. n= 32 biologically independent samples. CSM analyzed by paired two-tailed Student’s t -test, IgM + memory analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (D) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD + CD24 int CD38 int (mature) and CD19 + CD27 - IgD + CD24 hi CD38 hi transitional B cells ex vivo in paired BK and tumor. n= 32 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (E) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c + (DN2), CD19 + CD27 - IgD - CD21 - CD11c - (DN3), and CD19 + CD27 - IgD - CD21 + CD11c + (DN4) B cells ex vivo in BK and tumor; ** p <0.01. n= 18 biologically independent samples. Paired two-tailed Student’s t -test. Error bars represented as mean±SEM. (F) Cumulative data showing the frequencies of CD19 + CD27 - IgD + (naive), CD19 + CD27 + IgD - , CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c - (DN3) B cells, and CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) stratified according to intermediate (3-5) or high (>6) Leibovich scores; * p <0.05, ** p <0.01. For naive, CD27 + IgD - , and plasma cells n= 26, for DN1 and DN3 n= 13 biologically independent samples. Naive, DN1, DN3, and PC analyzed by unpaired two-tailed Student’s t -test, CD27 + IgD - analyzed by unpaired Mann-Whitney U test. Error bars represented as mean±SEM.
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    easyseptm human cd19 positive selection kit ii  (STEMCELL Technologies Inc)
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    (A) Representative contour plots and cumulative data showing the frequencies of <t>CD19</t> + CD27 - IgD - (double negative (DN)), CD19 + CD27 + IgD - , CD19 + CD27 + IgD + (unswitched memory (USM)) and CD19 + CD27 - IgD + (naive) B cells ex vivo in paired BK and matching tumor tissue; ** p <0.01, *** p <0.001. n= 32 biologically independent samples. DN, USM, and naive B cells analyzed by paired two-tailed Student’s t -test, CD19 + CD27 + IgD - B cells analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (B) Representative contour plots and cumulative data showing the frequencies of CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) and BLIMP-1 + B cells ex vivo in paired BK and matching tumor tissue. BLIMP-1 expression overlaid onto the B cell contour plot; ** p <0.01, **** p <0.0001. For plasma cells n =31 and for BLIMP-1 + B cells n =15 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (C) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 + IgD - CD24 + CD38 - IgM + (IgM + memory) and CD19 + CD27 + IgD - CD24 + CD38 - IgM - (class-switched memory (CSM)) B cells ex vivo in paired BK and tumor; *** p <0.001. n= 32 biologically independent samples. CSM analyzed by paired two-tailed Student’s t -test, IgM + memory analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (D) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD + CD24 int CD38 int (mature) and CD19 + CD27 - IgD + CD24 hi CD38 hi transitional B cells ex vivo in paired BK and tumor. n= 32 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (E) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c + (DN2), CD19 + CD27 - IgD - CD21 - CD11c - (DN3), and CD19 + CD27 - IgD - CD21 + CD11c + (DN4) B cells ex vivo in BK and tumor; ** p <0.01. n= 18 biologically independent samples. Paired two-tailed Student’s t -test. Error bars represented as mean±SEM. (F) Cumulative data showing the frequencies of CD19 + CD27 - IgD + (naive), CD19 + CD27 + IgD - , CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c - (DN3) B cells, and CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) stratified according to intermediate (3-5) or high (>6) Leibovich scores; * p <0.05, ** p <0.01. For naive, CD27 + IgD - , and plasma cells n= 26, for DN1 and DN3 n= 13 biologically independent samples. Naive, DN1, DN3, and PC analyzed by unpaired two-tailed Student’s t -test, CD27 + IgD - analyzed by unpaired Mann-Whitney U test. Error bars represented as mean±SEM.
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    Image Search Results


    (A) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - (double negative (DN)), CD19 + CD27 + IgD - , CD19 + CD27 + IgD + (unswitched memory (USM)) and CD19 + CD27 - IgD + (naive) B cells ex vivo in paired BK and matching tumor tissue; ** p <0.01, *** p <0.001. n= 32 biologically independent samples. DN, USM, and naive B cells analyzed by paired two-tailed Student’s t -test, CD19 + CD27 + IgD - B cells analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (B) Representative contour plots and cumulative data showing the frequencies of CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) and BLIMP-1 + B cells ex vivo in paired BK and matching tumor tissue. BLIMP-1 expression overlaid onto the B cell contour plot; ** p <0.01, **** p <0.0001. For plasma cells n =31 and for BLIMP-1 + B cells n =15 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (C) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 + IgD - CD24 + CD38 - IgM + (IgM + memory) and CD19 + CD27 + IgD - CD24 + CD38 - IgM - (class-switched memory (CSM)) B cells ex vivo in paired BK and tumor; *** p <0.001. n= 32 biologically independent samples. CSM analyzed by paired two-tailed Student’s t -test, IgM + memory analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (D) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD + CD24 int CD38 int (mature) and CD19 + CD27 - IgD + CD24 hi CD38 hi transitional B cells ex vivo in paired BK and tumor. n= 32 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (E) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c + (DN2), CD19 + CD27 - IgD - CD21 - CD11c - (DN3), and CD19 + CD27 - IgD - CD21 + CD11c + (DN4) B cells ex vivo in BK and tumor; ** p <0.01. n= 18 biologically independent samples. Paired two-tailed Student’s t -test. Error bars represented as mean±SEM. (F) Cumulative data showing the frequencies of CD19 + CD27 - IgD + (naive), CD19 + CD27 + IgD - , CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c - (DN3) B cells, and CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) stratified according to intermediate (3-5) or high (>6) Leibovich scores; * p <0.05, ** p <0.01. For naive, CD27 + IgD - , and plasma cells n= 26, for DN1 and DN3 n= 13 biologically independent samples. Naive, DN1, DN3, and PC analyzed by unpaired two-tailed Student’s t -test, CD27 + IgD - analyzed by unpaired Mann-Whitney U test. Error bars represented as mean±SEM.

    Journal: bioRxiv

    Article Title: Tumor-infiltrating CD27 - IgD - regulatory B cells suppress cytotoxic CD8 + T cell responses in renal cell carcinoma

    doi: 10.1101/2025.07.08.663720

    Figure Lengend Snippet: (A) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - (double negative (DN)), CD19 + CD27 + IgD - , CD19 + CD27 + IgD + (unswitched memory (USM)) and CD19 + CD27 - IgD + (naive) B cells ex vivo in paired BK and matching tumor tissue; ** p <0.01, *** p <0.001. n= 32 biologically independent samples. DN, USM, and naive B cells analyzed by paired two-tailed Student’s t -test, CD19 + CD27 + IgD - B cells analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (B) Representative contour plots and cumulative data showing the frequencies of CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) and BLIMP-1 + B cells ex vivo in paired BK and matching tumor tissue. BLIMP-1 expression overlaid onto the B cell contour plot; ** p <0.01, **** p <0.0001. For plasma cells n =31 and for BLIMP-1 + B cells n =15 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (C) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 + IgD - CD24 + CD38 - IgM + (IgM + memory) and CD19 + CD27 + IgD - CD24 + CD38 - IgM - (class-switched memory (CSM)) B cells ex vivo in paired BK and tumor; *** p <0.001. n= 32 biologically independent samples. CSM analyzed by paired two-tailed Student’s t -test, IgM + memory analyzed by paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (D) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD + CD24 int CD38 int (mature) and CD19 + CD27 - IgD + CD24 hi CD38 hi transitional B cells ex vivo in paired BK and tumor. n= 32 biologically independent samples. Paired two-tailed Wilcoxon test. Error bars represented as mean±SEM. (E) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c + (DN2), CD19 + CD27 - IgD - CD21 - CD11c - (DN3), and CD19 + CD27 - IgD - CD21 + CD11c + (DN4) B cells ex vivo in BK and tumor; ** p <0.01. n= 18 biologically independent samples. Paired two-tailed Student’s t -test. Error bars represented as mean±SEM. (F) Cumulative data showing the frequencies of CD19 + CD27 - IgD + (naive), CD19 + CD27 + IgD - , CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c - (DN3) B cells, and CD19 + CD24 lo/- CD38 hi (plasma cells (PCs)) stratified according to intermediate (3-5) or high (>6) Leibovich scores; * p <0.05, ** p <0.01. For naive, CD27 + IgD - , and plasma cells n= 26, for DN1 and DN3 n= 13 biologically independent samples. Naive, DN1, DN3, and PC analyzed by unpaired two-tailed Student’s t -test, CD27 + IgD - analyzed by unpaired Mann-Whitney U test. Error bars represented as mean±SEM.

    Article Snippet: Tissue-resident B cells were isolated using the EasySep immunomagnetic CD19 positive selection kit (STEMCELL, cat. no. 17854).

    Techniques: Ex Vivo, Two Tailed Test, Clinical Proteomics, Expressing, MANN-WHITNEY

    (A) Density UMAPs showing the distribution of IL10 hi , TGFB hi , TXN hi , AHR hi , HAVCR2 hi , TIGIT hi , CD274 hi and PDCD1 hi RCC-resident B cells. (B) Dotplot showing the proportion of cells expressing a Breg score comprising IL10, CD274, AHR, TXN, LAG3, TIGIT, CTLA4, CD86, TGFB1, and GZMB (dot size), and their average expression levels (color intensity) in the tumor, tumor margin, BK, and blood. (C) Spatial analysis of segmented CD20 + Bregs in a representative TLS. Staining of DAPI (dark blue) overlaid with cell segmentation (gating strategy shown in Figure S5A) of CD20 + IL-10 + Bregs (red), CD20 + TGFβ + Bregs (green), and CD20 + IL-10 + TGFβ + Bregs (orange). Scale bar = 100µm. (D) Cumulative data showing the frequencies of CD20 + IL-10 + Bregs, CD20 + TGFβ + Bregs, CD20 + IL-10 + TGFβ + within ROIs; * p <0.05, ** p <0.01. 13 ROIs across n =3 biologically independent samples. One-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM. (E) Spatial analysis of segmented CD138 + Bregs in a representative TLS. Staining of DAPI (dark blue) overlaid with cell segmentation (gating strategy shown in Figure S5A) of CD138 + IL-10 + Bregs (pink), CD138 + TGFβ + Bregs (dark green), and CD138 + IL-10 + TGFβ + Bregs (yellow) within TLSs. (F) Cumulative data showing the frequencies of CD138 + IL-10 + Bregs, CD20 + CD138 + TGFβ + Bregs, CD138 + IL-10 + TGFβ + Bregs within ROIs; * p <0.05, ** p <0.01. 13 ROIs across n =3 biologically independent samples. One-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM. (G) Cumulative data showing the frequencies of CD20 + CD27 - IgD + (naive), CD20 + CD27 + IgD + (unswitched memory (USM)), CD20 + CD27 + IgD - B cells, CD20 + CD27 - IgD - CD21 + CD11c - (DN1), CD20 + CD27 - IgD - CD21 - CD11c + (DN2), CD20 + CD27 - IgD - CD21 - CD11c - (DN3), and CD20 + CD27 - IgD - CD21 + CD11c + (DN4) within CD20 + IL-10 + Bregs; * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001. 13 ROIs across n =3 biologically independent samples. Fridman test with Dunn’s test for multiple comparisons. Error bars represented as mean±SEM. (H) Cumulative data showing the frequencies of CD20 + CD27 - IgD + (naive), CD20 + CD27 + IgD + (unswitched memory (USM)), CD20 + CD27 - IgD - (double negative (DN)), CD20 + CD27 + IgD - B cells, CD20 + CD27 - IgD - CD21 + CD11c - (DN1), CD20 + CD27 - IgD - CD21 - CD11c + (DN2), CD20 + CD27 - IgD - CD21 - CD11c - (DN3), and CD20 + CD27 - IgD - CD21 + CD11c + (DN4) B cells within CD20 + TGFβ + Bregs; * p <0.05, ** p <0.01. 13 ROIs across n =3 biologically independent samples. Fridman test with Dunn’s test for multiple comparisons. Error bars represented as mean±SEM. (I) Prognostic association of high versus low CD19, IL-10, TGFB1 signature with survival in RCC using The Cancer Genome Atlas (TCGA) data. p values were derived by Cox proportional hazard model adjusting for signature group, age, sex, and American Joint Committee on Cancer (AJCC) stage.

    Journal: bioRxiv

    Article Title: Tumor-infiltrating CD27 - IgD - regulatory B cells suppress cytotoxic CD8 + T cell responses in renal cell carcinoma

    doi: 10.1101/2025.07.08.663720

    Figure Lengend Snippet: (A) Density UMAPs showing the distribution of IL10 hi , TGFB hi , TXN hi , AHR hi , HAVCR2 hi , TIGIT hi , CD274 hi and PDCD1 hi RCC-resident B cells. (B) Dotplot showing the proportion of cells expressing a Breg score comprising IL10, CD274, AHR, TXN, LAG3, TIGIT, CTLA4, CD86, TGFB1, and GZMB (dot size), and their average expression levels (color intensity) in the tumor, tumor margin, BK, and blood. (C) Spatial analysis of segmented CD20 + Bregs in a representative TLS. Staining of DAPI (dark blue) overlaid with cell segmentation (gating strategy shown in Figure S5A) of CD20 + IL-10 + Bregs (red), CD20 + TGFβ + Bregs (green), and CD20 + IL-10 + TGFβ + Bregs (orange). Scale bar = 100µm. (D) Cumulative data showing the frequencies of CD20 + IL-10 + Bregs, CD20 + TGFβ + Bregs, CD20 + IL-10 + TGFβ + within ROIs; * p <0.05, ** p <0.01. 13 ROIs across n =3 biologically independent samples. One-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM. (E) Spatial analysis of segmented CD138 + Bregs in a representative TLS. Staining of DAPI (dark blue) overlaid with cell segmentation (gating strategy shown in Figure S5A) of CD138 + IL-10 + Bregs (pink), CD138 + TGFβ + Bregs (dark green), and CD138 + IL-10 + TGFβ + Bregs (yellow) within TLSs. (F) Cumulative data showing the frequencies of CD138 + IL-10 + Bregs, CD20 + CD138 + TGFβ + Bregs, CD138 + IL-10 + TGFβ + Bregs within ROIs; * p <0.05, ** p <0.01. 13 ROIs across n =3 biologically independent samples. One-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM. (G) Cumulative data showing the frequencies of CD20 + CD27 - IgD + (naive), CD20 + CD27 + IgD + (unswitched memory (USM)), CD20 + CD27 + IgD - B cells, CD20 + CD27 - IgD - CD21 + CD11c - (DN1), CD20 + CD27 - IgD - CD21 - CD11c + (DN2), CD20 + CD27 - IgD - CD21 - CD11c - (DN3), and CD20 + CD27 - IgD - CD21 + CD11c + (DN4) within CD20 + IL-10 + Bregs; * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001. 13 ROIs across n =3 biologically independent samples. Fridman test with Dunn’s test for multiple comparisons. Error bars represented as mean±SEM. (H) Cumulative data showing the frequencies of CD20 + CD27 - IgD + (naive), CD20 + CD27 + IgD + (unswitched memory (USM)), CD20 + CD27 - IgD - (double negative (DN)), CD20 + CD27 + IgD - B cells, CD20 + CD27 - IgD - CD21 + CD11c - (DN1), CD20 + CD27 - IgD - CD21 - CD11c + (DN2), CD20 + CD27 - IgD - CD21 - CD11c - (DN3), and CD20 + CD27 - IgD - CD21 + CD11c + (DN4) B cells within CD20 + TGFβ + Bregs; * p <0.05, ** p <0.01. 13 ROIs across n =3 biologically independent samples. Fridman test with Dunn’s test for multiple comparisons. Error bars represented as mean±SEM. (I) Prognostic association of high versus low CD19, IL-10, TGFB1 signature with survival in RCC using The Cancer Genome Atlas (TCGA) data. p values were derived by Cox proportional hazard model adjusting for signature group, age, sex, and American Joint Committee on Cancer (AJCC) stage.

    Article Snippet: Tissue-resident B cells were isolated using the EasySep immunomagnetic CD19 positive selection kit (STEMCELL, cat. no. 17854).

    Techniques: Expressing, Staining, Derivative Assay

    (A) Radar plot showing the Human Molecular Signatures Database and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enriched in each tumor-infiltrating B cell (TIB) cluster. (B) Density UMAPs showing the distribution of TLR7 hi , TLR9 hi , MYD88 hi , IRAK1 hi , IRAK4 hi , TRAF6 hi , MAP3K7 hi , and NFKB1 hi RCC-resident B cells. (C) Representative contour plots and cumulative data showing IL-10 expression by tumor-infiltrating CD19 + B cells ex vivo (white) and following 72h stimulation with R848 (light grey) or CpGC (dark grey); ** p <0.01, *** p <0.001. For ex vivo n =16, R848 and CpGC n =22 biologically independent samples. Mixed-effects one-way ANOVA with Dunnett’s test for multiple comparisons. Error bars represented as mean±SEM. (D) Representative contour plots and cumulative data showing TGFβ expression by tumor-infiltrating CD19 + B cells ex vivo (white) and following 72h stimulation with R848 (light grey) or CpGC (dark grey); * p <0.05, ** p <0.01. For ex vivo n =13, R848 and CpGC n =10 biologically independent samples. One-way Fridman test with Dunn’s test for multiple comparisons. Error bars represented as mean±SEM. (E) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD + (naive), CD19 + CD27 + IgD + (unswitched memory (USM)), CD19 + CD27 + IgD - , and CD19 + CD27 - IgD - (double negative (DN)) B cells within tumor-infiltrating CD19 + IL-10 + B cells, ex vivo and following 72h stimulation with R848 or CpGC; * p <0.05, ** p <0.01, **** p <0.0001. n =24 biologically independent samples. Two-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM. (F) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c + (DN2), CD19 + CD27 - IgD - CD21 - CD11c - (DN3) and CD19 + CD27 - IgD - CD21 + CD11c + (DN4) B cells within tumor-infiltrating CD19 + IL-10 + B cells, ex vivo and following 72h stimulation with R848 or CpGC; * p <0.05, *** p <0.001, **** p <0.0001. n =18 biologically independent samples. Two-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM. (G) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD + (naive), CD19 + CD27 + IgD + (unswitched memory (USM)), CD19 + CD27 + IgD - , and CD19 + CD27 - IgD - (double negative (DN)) B cells within tumor-infiltrating CD19 + TGFβ + B cells, ex vivo and following 72h stimulation with R848 or CpGC; ** p <0.01, **** p <0.0001. n =12 biologically independent samples. Two-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM. (H) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c + (DN2), CD19 + CD27 - IgD - CD21 - CD11c - (DN3) and CD19 + CD27 - IgD - CD21 + CD11c + (DN4) B cells within tumor-infiltrating CD19 + TGFβ + B cells, ex vivo and following 72h stimulation with R848 or CpGC; *** p <0.001, **** p <0.0001. n =11 biologically independent samples. Two-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM.

    Journal: bioRxiv

    Article Title: Tumor-infiltrating CD27 - IgD - regulatory B cells suppress cytotoxic CD8 + T cell responses in renal cell carcinoma

    doi: 10.1101/2025.07.08.663720

    Figure Lengend Snippet: (A) Radar plot showing the Human Molecular Signatures Database and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enriched in each tumor-infiltrating B cell (TIB) cluster. (B) Density UMAPs showing the distribution of TLR7 hi , TLR9 hi , MYD88 hi , IRAK1 hi , IRAK4 hi , TRAF6 hi , MAP3K7 hi , and NFKB1 hi RCC-resident B cells. (C) Representative contour plots and cumulative data showing IL-10 expression by tumor-infiltrating CD19 + B cells ex vivo (white) and following 72h stimulation with R848 (light grey) or CpGC (dark grey); ** p <0.01, *** p <0.001. For ex vivo n =16, R848 and CpGC n =22 biologically independent samples. Mixed-effects one-way ANOVA with Dunnett’s test for multiple comparisons. Error bars represented as mean±SEM. (D) Representative contour plots and cumulative data showing TGFβ expression by tumor-infiltrating CD19 + B cells ex vivo (white) and following 72h stimulation with R848 (light grey) or CpGC (dark grey); * p <0.05, ** p <0.01. For ex vivo n =13, R848 and CpGC n =10 biologically independent samples. One-way Fridman test with Dunn’s test for multiple comparisons. Error bars represented as mean±SEM. (E) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD + (naive), CD19 + CD27 + IgD + (unswitched memory (USM)), CD19 + CD27 + IgD - , and CD19 + CD27 - IgD - (double negative (DN)) B cells within tumor-infiltrating CD19 + IL-10 + B cells, ex vivo and following 72h stimulation with R848 or CpGC; * p <0.05, ** p <0.01, **** p <0.0001. n =24 biologically independent samples. Two-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM. (F) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c + (DN2), CD19 + CD27 - IgD - CD21 - CD11c - (DN3) and CD19 + CD27 - IgD - CD21 + CD11c + (DN4) B cells within tumor-infiltrating CD19 + IL-10 + B cells, ex vivo and following 72h stimulation with R848 or CpGC; * p <0.05, *** p <0.001, **** p <0.0001. n =18 biologically independent samples. Two-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM. (G) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD + (naive), CD19 + CD27 + IgD + (unswitched memory (USM)), CD19 + CD27 + IgD - , and CD19 + CD27 - IgD - (double negative (DN)) B cells within tumor-infiltrating CD19 + TGFβ + B cells, ex vivo and following 72h stimulation with R848 or CpGC; ** p <0.01, **** p <0.0001. n =12 biologically independent samples. Two-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM. (H) Representative contour plots and cumulative data showing the frequencies of CD19 + CD27 - IgD - CD21 + CD11c - (DN1), CD19 + CD27 - IgD - CD21 - CD11c + (DN2), CD19 + CD27 - IgD - CD21 - CD11c - (DN3) and CD19 + CD27 - IgD - CD21 + CD11c + (DN4) B cells within tumor-infiltrating CD19 + TGFβ + B cells, ex vivo and following 72h stimulation with R848 or CpGC; *** p <0.001, **** p <0.0001. n =11 biologically independent samples. Two-way ANOVA with Tukey’s test for multiple comparisons. Error bars represented as mean±SEM.

    Article Snippet: Tissue-resident B cells were isolated using the EasySep immunomagnetic CD19 positive selection kit (STEMCELL, cat. no. 17854).

    Techniques: Expressing, Ex Vivo